Journal
LANGMUIR
Volume 25, Issue 12, Pages 7169-7176Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la9001972
Keywords
-
Funding
- National Science and Engineering Research Council of Canada
- Canadian Foundation for Innovation
Ask authors/readers for more resources
Plastic substrates made of polystyrene (PS) and Zeonor 1060R were treated with oxygen plasma to introduce polar groups (e.g., carbonyl and carboxylic acid) at the surface that render these materials hydrophilic and promote patterned adhesion of HeLa cells. Resultant surfaces were characterized using contact angle goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) to monitor changes in wettability, nanoscale topography, and chemical composition. Biocompatibility of the plastic surfaces was verified through fluorescence microscopy using three fluorophores, Alexa Fluor 488 conjugated to Annexin V, Hoechst 33258, and propidium iodide, indicating cells that undergo apoptosis and necrosis, respectively. The best cell growth was observed on PS treated at 5 W/sccm, for which the viability of adhering HeLa cells exceeded 90%. Patterning was accomplished using an elastomeric microcapillary system (mu CS) made of poly(dimethylsiloxane) (PDMS) that consisted of a set of parallel channels to align cells in linear fashion. Densely copulated bands were obtained on substrates of both plastic materials when the culture medium contained >2 x 10(5) cells/mL.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available