Journal
LANGMUIR
Volume 24, Issue 2, Pages 563-567Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la7026626
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Protein assays are critical analytical tools performed in various biochemical laboratories to quantity the concentration of proteinns. In this study, we report the optical responses of a thin layer of liquid crystals supported on glass slides decorated with proteins and the utility Of this phenomenon as a new all-or-nothing type of protein assay. It was found that the orientations of liquid crystals are very sensitive to the concentration of protein solution applied to the surface. When the protein concentration exceeds a critical value (IgG 5.0 mu g/mL, BSA 6.0 mu g/mL, FTIC-anti-biotin 0.40 mu g/mL, and FITC-anti-IgG 0.37 mu g/mL), the thin layer of liquid crystals gives a very sharp dark-to-bright optical response within a small concentration range. This characteristic is not observed in any traditional protein assays, which are based on the adsorption of UV or visible light. The optical response is also very precise and reproducible. It is not affected by the thickness of the liquid crystal cell or the amount of organosilanes coated on the glass slides. The liquid crystal-based protein assay may be very useful for screening purposes, especially when a simple positive or negative answer is desired.
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