Journal
LANGMUIR
Volume 24, Issue 7, Pages 3429-3433Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la702673p
Keywords
-
Funding
- NCRR NIH HHS [RR-08119, P41 RR008119] Funding Source: Medline
- NHGRI NIH HHS [HG-002655, R01 HG002655-03, R01 HG002655-05, R01 HG002655-04A1, R01 HG002655] Funding Source: Medline
- NIBIB NIH HHS [R01 EB000682, R01 EB006521, EB0065211, R01 EB006521-02, R01 EB006521-01A1, EB000682] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR008119] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG002655] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000682, R01EB006521] Funding Source: NIH RePORTER
Ask authors/readers for more resources
The fluorescence signal of single organic fluorophores is characterized by random blinking and irreversible photobleaching. Photoiriduced blinking of Cy5 has posed various limitations of this popular near-infrared (NIR) probe in biological applications. Here we show that fluorophore-metal nanoparticle (NP) complexes greatly suppress Cy5 blinking and noticeably reduce photobleaching events. The blinking behavior of single Cy5 molecules was investigated and compared in the absence and the presence of silver nanostructures. A power-law distribution of off time population was observed for single Cy5 molecules. Average off times were compared to evaluate the plasmonic effect of silver nanoparticles oil the triplet decay rates. We furthermore demonstrate enhanced photostability in the presence of silver NPs. The results show that plasmonic-control led fluorescence can lead to a novel physical mechanism to enhance fluorescence intensity, reduce blinking, and increase photostability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available