Journal
PURINERGIC SIGNALLING
Volume 11, Issue 4, Pages 595-606Publisher
SPRINGER
DOI: 10.1007/s11302-015-9478-z
Keywords
Adenosine A(1) receptors; ATP catabolism; Acetylcholine release; Ectonucleotidases; Human urinary bladder; Bladder outlet obstruction
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT, FEDER) [PTDC/SAU-OSM/104369/2008, REEQ/1168/SAU/2005, REEQ/1264/SAU/2005, PEst-OE/SAU/UI0215/2014]
- Associacao Portuguesa de Urologia (APU)
- University of Porto/Caixa Geral de Depositos (Investigacao Cientifica na Pre-Graduacao)
- FCT [SFRH/BD/88855/2012]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/88855/2012, PTDC/SAU-OSM/104369/2008] Funding Source: FCT
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This study was designed to investigate whether reduced adenosine formation linked to deficits in extracellular ATP hydrolysis by NTPDases contributes to detrusor neuromodulatory changes associated with bladder outlet obstruction in men with benign prostatic hyperplasia (BPH). The kinetics of ATP catabolism and adenosine formation as well as the role of P1 receptor agonists on muscle tension and nerve-evoked [H-3]ACh release were evaluated in mucosal-denuded detrusor strips from BPH patients (n = 31) and control organ donors (n = 23). The neurogenic release of ATP and [H-3]ACh was higher (P < 0.05) in detrusor strips from BPH patients. The extracellular hydrolysis of ATP and, subsequent, adenosine formation was slower (t (1/2) 73 vs. 36 min, P < 0.05) in BPH detrusor strips. The A(1) receptor-mediated inhibition of evoked [H-3]ACh release by adenosine (100 mu M), NECA (1 mu M), and R-PIA (0.3 mu M) was enhanced in BPH bladders. Relaxation of detrusor contractions induced by acetylcholine required 30-fold higher concentrations of adenosine. Despite VAChT-positive cholinergic nerves exhibiting higher A(1) immunoreactivity in BPH bladders, the endogenous adenosine tonus revealed by adenosine deaminase is missing. Restoration of A(1) inhibition was achieved by favoring (1) ATP hydrolysis with apyrase (2 U mL(-1)) or (2) extracellular adenosine accumulation with dipyridamole or EHNA, as these drugs inhibit adenosine uptake and deamination, respectively. In conclusion, reduced ATP hydrolysis leads to deficient adenosine formation and A(1) receptor-mediated inhibition of cholinergic nerve activity in the obstructed human bladder. Thus, we propose that pharmacological manipulation of endogenous adenosine levels and/or A(1) receptor activation might be useful to control bladder overactivity in BPH patients.
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