Journal
LABORATORY INVESTIGATION
Volume 93, Issue 5, Pages 611-621Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/labinvest.2013.44
Keywords
breast cancer; cell surface transportome; flow cytometry analysis; live cells; metabolite transporters; tumor dissociation; xenografts
Categories
Funding
- INCa/DGOS program
- ANR Glutstem
- AFM
- FRM
- ARC
- European Community [LSHC-CT-2005-018914]
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Metabolic adaptations and changes in the expression of nutrient transporters are known to accompany tumorigenic processes. Nevertheless, in the context of solid tumors, studies of metabolism are hindered by a paucity of tools allowing the identification of cell surface transporters on individual cells. Here, we developed a method for the dissociation of human breast cancer tumor xenografts combined with quantification of cell surface markers, including metabolite transporters. The expression profiles of four relevant nutrient transporters for cancer cells' metabolism, Glut1, ASCT2, PiT1 and PiT2 (participating to glucose, glutamine and inorganic phosphate, respectively), as detected by new retroviral envelope glycoprotein-derived ligands, were distinctive of each tumor, unveiling underlying differences in metabolic pathways. Our tumor dissociation procedure and nutrient transporter profiling technology provides opportunities for future basic research, clinical diagnosis, prognosis and evaluation of therapeutic responses, as well as for drug discovery and development. Laboratory Investigation (2013) 93, 611-621; doi:10.1038/labinvest.2013.44; published online 4 March 2013
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