Journal
LABORATORY INVESTIGATION
Volume 90, Issue 11, Pages 1645-1660Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/labinvest.2010.149
Keywords
astrocyte; basement membrane; blood-brain barrier; DAPs; dystrophy
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Funding
- Fondazione Cassa di Risparmio di Puglia, Bari, Italy
- MIUR, Rome [PRIN 2007]
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In this study, we investigated the involvement of dystrophin-associated proteins (DAPs) and their relationship with the perivascular basement membrane in the brains of mdx mice and controls at the age of 2 months. We analyzed (1) the expression of glial DAPs alpha-beta-dystroglycan (DG), alpha-syntrophin, aquaporin-4 (AQP4) water channel, Kir 4.1 and dystrophin isoform (Dp71) by immunocytochemistry, laser confocal microscopy, immunogold electron microscopy, immunoblotting and RT-PCR; (2) the ultrastructure of the basement membrane and expression of laminin and agrin; and (3) the dual immunofluorescence colocalization of AQP4/alpha-beta-DG, and of Kir 4.1/agrin. The following results were observed in mdx brain as compared with controls: (1) a significant reduction in protein content and mRNA expression of DAPs; (2) ultrastructurally, a thickened and discontinuous appearance of the basement membrane and a significant reduction in laminin and agrin; and (3) a molecular rearrangment of alpha-beta-DG, coupled with a parallel loss of agrin and Kir 4.1 on basement membrane and glial endfeet. These data indicate that in mdx brain the deficiency in dystrophin and dystrophin isoform (Dp71) is coupled with a reduction of DAP components, coupled with an altered anchoring to the basement membrane. Laboratory Investigation (2010) 90, 1645-1660; doi:10.1038/labinvest.2010.149; published online 16 August 2010
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