Journal
LAB ON A CHIP
Volume 14, Issue 15, Pages 2651-2656Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc51427g
Keywords
-
Categories
Funding
- European Research Council (ERC) [246812 Intercom]
- Netherlands Organization for Scientific Research (NWO)
- Ministry of Education, Culture and Science [024.001.035]
Ask authors/readers for more resources
We microfluidically fabricate bio-orthogonal DNA-functionalized porous hydrogels from hyaluronic acid that are employed in in vitro transcription/translation (IVTT) of a green fluorescent protein. By co-encapsulating individual hydrogel particles and the IVTT machinery in water-in-oil microdroplets, we study protein expression in a defined reaction volume. Our approach enables precise control over protein expression rates by gene dosage. We show that gene transcription and translation are confined to the membrane-free hydrogel matrix, which contributes to the design of membrane-free protocells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available