Journal
LAB ON A CHIP
Volume 14, Issue 23, Pages 4447-4450Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc00701h
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Funding
- NIA NIH HHS [R21 AG041498, R01 AG046358] Funding Source: Medline
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We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes.
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