Journal
LAB ON A CHIP
Volume 14, Issue 22, Pages 4338-4343Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc00780h
Keywords
-
Categories
Funding
- FEMTOSTACK [ANR-2010-BLAN-1529-01]
- Region Alsace
- Institut Carnot MICA
Ask authors/readers for more resources
We demonstrate time-correlated single photon counting (TCSPC) in microfluidic droplets under high-throughput conditions. We discuss the fundamental limitations in the photon acquisition rate imposed by the single photon detection technique and show that it does not preclude accurate fluorescence lifetime (FLT) measurements at a droplet throughput exceeding 1 kHz with remarkable sensitivity. This work paves the way for the implementation of innovative biomolecular interaction assays relying on the FLT detection of nanosecond-lived fluorophores for high-throughput biotechnological applications, including high-throughput screening or cell sorting potentially allowed by droplet microfluidics or other fast sample handling facilities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available