Journal
LAB ON A CHIP
Volume 14, Issue 12, Pages 1991-1995Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc00241e
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Funding
- FCT for his PhD grant [SFRH/BD/74037/2010]
- Fundacao para a Ciencia e a Tecnologia (FCT) through project Alfa-Syn [PTDC/BIABCM/117975/2010]
- Associated Laboratory IN - Institute of Nanoscience and Nanotechnology [PEst-OE/CTM/LA0024/2013]
- Fundação para a Ciência e a Tecnologia [PEst-OE/CTM/LA0024/2013] Funding Source: FCT
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Microfabricated amorphous silicon photodiodes were integrated with prism-like PDMS microfluidics for the detection and quantification of fluorescence signals. The PDMS device was fabricated with optical quality surfaces and beveled sides. A 405 nm laser beam perpendicular to the lateral sides of the microfluidic device excites the fluorophores in the microchannel at an angle of 70 degrees to the normal to the microchannel/photodiode surface. This configuration, which makes use of the total internal reflection of the excitation beam and the isotropy of the fluorescence emission, minimizes the intensity of excitation light that reaches the integrated photodetector. A difference of two orders of magnitude was achieved in the reduction of the detection noise level as compared with a normally incident excitation configuration. A limit-of-detection of 5.6 x 10(10) antibodies per square centimeter was achieved using antibodies labeled with a model organic fluorophore. Furthermore, the results using the lateral excitation scheme are in good proportionality agreement with those by fluorescence quantification using wide-field fluorescence microscopy.
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