Journal
LAB ON A CHIP
Volume 13, Issue 12, Pages 2278-2284Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc50168j
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Funding
- NSF CAREER AWARD [ECCS 0747950, ECCS-1232279, DBI 0852701]
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- ARO Young Investigator Award
- NSF CAREER Award (Biophotonics program)
- ONR Young Investigator Award
- NIH from the Office of The Director [DP2OD006427]
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP2OD006427] Funding Source: NIH RePORTER
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We demonstrate an optoelectronic tweezer (OET) coupled to a lensfree holographic microscope for real-time interactive manipulation of cells and micro-particles over a large field-of-view (FOV). This integrated platform can record the holographic images of cells and particles over the entire active area of a CCD sensor array, perform digital image reconstruction to identify target cells, dynamically track the positions of cells and particles, and project light beams to trigger light-induced dielectrophoretic forces to pattern and sort cells on a chip. OET technology has been previously shown to be capable of performing parallel single cell manipulation over a large area. However, its throughput has been bottlenecked by the number of cells that can be imaged within the limited FOV of a conventional microscope objective lens. Integrating lensfree holographic imaging with OET solves this fundamental FOV barrier, while also creating a compact on-chip cell/particle manipulation platform. Using this unique platform, we have successfully demonstrated real-time interactive manipulation of thousands of single cells and micro-particles over an ultra-large area of e. g., 240 mm(2) (i.e. 17.96 mm x 13.52 mm).
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