Journal
LAB ON A CHIP
Volume 13, Issue 1, Pages 68-72Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40948h
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Funding
- Italian MiUR [PRIN2008/20088L3BP3, IRB-MERIT/RBNE08HWLZ010, PONR&C2007-2013/PON02_00355_3416798]
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Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.
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