Journal
LAB ON A CHIP
Volume 13, Issue 22, Pages 4366-4373Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc50778e
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Funding
- European Union
- IMI
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We present a method for efficient air bubble removal in microfluidic applications. Air bubbles are extracted from a liquid chamber into a vacuum chamber through a semipermeable membrane, consisting of PDMS coated with amorphous Teflon (R) AF 1600. Whereas air is efficiently extracted through the membrane, water loss is greatly reduced by the Teflon even at elevated temperatures. We present the water loss and permeability change with the amount of added Teflon AF to the membrane. Also, we demonstrate bubble-free, multiplex DNA amplification using PCR in a PDMS microfluidic device.
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