Journal
LAB ON A CHIP
Volume 13, Issue 7, Pages 1316-1324Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40364a
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Funding
- FSU Research Foundation GAP award
- Florida Department of Health Bankhead-Coley Cancer Research Program Technology Transfer Feasibility award [1BF03-43278]
- National Institute for General Medical Sciences grant [PO1 GM085354]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P01GM085354] Funding Source: NIH RePORTER
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DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. To address this limitation, we have developed a novel FISH sample-preparation method based on generating a centimetre-sized cell array, in which all cells are precisely positioned and separated from their neighbours. This method is simple and capable of patterning nonadherent human cells. We have successfully performed DNA FISH on the single-cell arrays, which facilitates analysis of the FISH results with the FISH-FINDER computer program.
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