Journal
LAB ON A CHIP
Volume 13, Issue 3, Pages 349-354Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40993c
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Funding
- U.S. Defense Threat Reduction Agency [HDTRA1-11-1-0005]
- Department of Defense (DoD) through the Air Force Office of Scientific Research, National Defense Science and Engineering Graduate Fellowship (NDSEG) [32 CFR 168a]
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We report on real-time electrochemical detection of individual DNA hybridization events at an electrode surface. The experiment is carried out in a microelectrochemical device configured with a working electrode modified with single-stranded DNA probe molecules. When a complementary DNA strand labelled with a catalyst hybridizes to the probe, an easily detectable electrocatalytic current is observed. In the experiments reported here, the catalyst is a platinum nanoparticle and the current arises from electrocatalytic oxidation of hydrazine. Two types of current transients are observed: short bursts and longer-lived steps. At low concentrations of hydrazine, the average size of the current transients is proportional to the amount of hydrazine present, but at higher concentrations the hydrazine oxidation reaction interferes with hybridization.
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