Journal
LAB ON A CHIP
Volume 12, Issue 6, Pages 1102-1109Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc20588b
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Funding
- Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) [R0A-2008-000-20078-0]
- Korea CCS RD Center
- Advanced Biomass R&D Center (ABC) of Korea [2010-0029728]
- Ministry of Science and Technology of Korean government of the Republic of Korea
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We report a simple, ultra-sensitive, and straightforward method for non-labeling detection of a cancer biomarker, using Rayleigh light scattering spectroscopy of the individual nanosensor based on antibody-antigen recognition and localized surface plasmon resonance (LSPR) lambda(max) shifts. By experimentally measuring the refractive index sensitivity of Au nanorods, the Au nanorod with an aspect ratio of similar to 3.5 was proven optimal for the LSPR sensing. To reduce the steric hindrance effect as well as to immobilize a large amount of ligand on the nanoparticle surface, various mixtures containing different molar ratios of HS(CH2)(11)(OCH2CH2)(6)OCH2COOH and HS(CH2)(11)(OCH2CH2)(3)OH were applied to form different self-assembled monolayer surfaces. The results showed that the best molar ratio for antibody conjugation was 1 : 10. When using individual Au nanorod sensors for the detection of prostate specific antigen (PSA), the lowest concentration recorded was similar to 1 aM (similar to 6 x 10(5) molecules), corresponding to LSPR lambda max shifts of similar to 4.2 nm. These results indicate that sensor miniaturization down to the nanoscale level, the reduction of steric hindrance, and optimization of size, shape, and aspect ratio of nanorods have led to a significant improvement in the detection limit of sensors.
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