Journal
LAB ON A CHIP
Volume 12, Issue 18, Pages 3341-3347Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40537g
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Funding
- DARPA (Micro/Nano Fluidics Fundamentals Focus Centre)
- NIH [R01CA155305, U54CA151838]
- NSF [0967375, 0546012]
- NATIONAL CANCER INSTITUTE [R01CA155305, U54CA151838] Funding Source: NIH RePORTER
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In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification abilities of the platform are tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform sample-to-answer'' pathogen detection of single cells is demonstrated using E. coli as a model pathogen.
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