Journal
LAB ON A CHIP
Volume 12, Issue 3, Pages 659-661Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc20515g
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Funding
- National Institutes of Health [HL092836, DE019024, EB012597, AR057837, DE021468, HL099073, EB008392]
- National Science Foundation [DMR0847287]
- Office of Naval Research
- US Army Corps of Engineers
- Progetto Rocca Visiting PhD Student Fellowship
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0847287] Funding Source: National Science Foundation
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This technical note describes a new bench-top method for producing anisotropic hydrogels composed of gradient layers of soluble factors, particles, polymer concentrations or material properties. Each gradient layer was produced by a previous gradient method in which a droplet of one precursor solution was added to a thin layer of a second solution. The ensuing rapid capillary flow along the open channel generated a gradient precursor solution, which was then crosslinked to form a gradient gel. Repeating these steps allowed a layered gel to be iteratively constructed with as many gradient layers as desired. This technique renders the synthesis of multi-layered gradient gels accessible to virtually any researcher and should help simplify the production of more biologically relevant cellular microenvironments.
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