Journal
LAB ON A CHIP
Volume 12, Issue 20, Pages 4037-4040Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40801e
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Funding
- University of Maryland at College Park
- NIDCR
- NIH [DE 019524]
- National Kidney Foundation of the National Capital Area
- China Scholarship Council
- Institute for Clinical Research Inc., Washington DC
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R03DE019524] Funding Source: NIH RePORTER
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This communication describes the formation of tubular structures with a circular cross-section by growing epithelial cells in a microfluidic (MF) device. Here we show for the first time that it is possible to form a monolayer of polarized cells, embedded within the MF device which can function as an in vivo epithelia. We showed: i) the overexpression of specific protein(s) of interest (i. e., ion channel and transport proteins) is feasible inside tubular structures in MFs; ii) the functional kinetic information of Ca2+ in cells can be measured by microflurometry using cell permeable Ca2+ probe under confocal microscope; and iii) calcium phosphate stones can be produced in real time in MFs with Ca2+ transporting epithelia. These data suggest that tubular structures inside this MF platform can be used as a suitable model to understand the molecular and pharmacological basis of calcium phosphate stone formation in the epithelial or other similar cellular micro environments.
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