Journal
LAB ON A CHIP
Volume 12, Issue 17, Pages 3184-3189Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40322f
Keywords
-
Categories
Funding
- National Basic Research Program of China (973 Program) [2011CB911003]
- National High-Tech Development Plan (863 Program) [2009AA093701]
- National Natural Foundation of China [20975050, 21175066]
- Jiangsu Province Science and Technology Support Program [BE2011773]
- National Science Funds for Creative Research Groups [21121091]
Ask authors/readers for more resources
In the present work we design a novel aptamer-based silver nanosensor for one-spot simultaneously detection of multiple proteins. SS-DNA modified AgNPs were immobilized on the aldehyde coated glass slide to form an AgNP array. Then dye-labeled aptamer sequences were allowed to hybridize with their complementary strands assembled on the surface of AgNPs. The target proteins were introduced to associate with the corresponding aptamers to form the aptamer-target complexes. The removal of the aptamer-target complexes resulted in a remarkable decrease in fluorescent signals. This nanosensor is found to be highly sensitive for the detection of proteins. When thrombin was employed as a sample model, the limit of detection of the optimized nanosenor was 0.4 fmol with a linear response of 0.8 fmol to 0.5 pmol. We further demonstrated the multiple protein detection of IgE and thrombin using multicolor silver nanoprobes, which shows effective recognition of the relative protein individually or simultaneously. This silver nanosensor offers a unique heterogeneous approach for protein detection with several advantages, such as high sensitivity, rapidity, high throughput, and miniaturization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available