Journal
LAB ON A CHIP
Volume 12, Issue 2, Pages 317-324Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20845d
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Funding
- National Natural Science Foundation of China [20733001, 20890020, 90913011, 20905004]
- Ministry of Science and Technology of China [2007CB714502, 2009AA04Z309, 2011CB809106]
- Ministry of Education of China
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Immunofluorescence (IF) is a common method to observe protein distribution and localization at the single-cell level through wide-field fluorescence or confocal microscopy. Conventional protocol for IF staining of cells typically requires a large amount of reagents, especially antibodies, and noticeable investment in both labor and time. Microfluidic technologies provide a cost-effective alternative: it can evaluate and optimize experimental conditions, and perform automatic and high-throughput IF staining on-chip. We employed this method to analyze lysosomal storage disorders (LSDs) based on the expression and morphological distribution of LAMP1 and LC3 in starving cells. With pneumatic valves integrated on-chip, the parallel staining process can be completed within a few hours. The total consumption of each antibody solution for the whole experiment is merely 0.3 mu L. This device provides a promising tool for automated high-throughput molecular imaging at cell level that can be applied for diagnostic analysis.
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