Journal
LAB ON A CHIP
Volume 11, Issue 6, Pages 1065-1073Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00472c
Keywords
-
Categories
Funding
- Singapore-MITAlliance for Research and Technology (SMART) Center
- National Institutes of Health [R01 HL094270-01A1, 1-R01-GM076689-01]
- NIEHS [5-T32-ES007020]
- MIT Startup Funds
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL094270] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [T32ES007020] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076689] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated deformability cytometer'' that measures dynamic mechanical responses of 10(3) to 10(4) individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available