Journal
LAB ON A CHIP
Volume 11, Issue 16, Pages 2763-2771Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20257j
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Funding
- European Community [FP7/2007-2013, HEALTH-F5-2008-201619]
- German Research Foundation [WE3737/3-1]
- German Federal Ministry of Education and Research [0101-31P6541]
- Ministry of Innovation, Science, Research and Technology of the State of North Rhine-Westphalia
- International Leibniz Graduate School Systems Biology Lab-on-a-Chip''
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Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 mu m track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).
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