Journal
LAB ON A CHIP
Volume 11, Issue 7, Pages 1256-1261Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00613k
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Funding
- UK Engineering and Physical Sciences Research Council [EP/C54269X/1, EP/G00465X/1]
- Institute of Chemical Biology
- Engineering and Physical Sciences Research Council [EP/G00465X/1, EP/C54269X/1] Funding Source: researchfish
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We have developed a generic platform to undertake the analysis of protein copy number from single cells. The approach described here is 'all-optical' whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.
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