Journal
LAB ON A CHIP
Volume 11, Issue 10, Pages 1721-1729Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00680g
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Funding
- National Science Foundation, Nanoscale Science and Engineering Center (NSEC
- USA)
- National Institutes of Health, Human Genome Research Institute (NHGRI
- USA)
- MEST [2010-0015392, 2010-0028226, 2010K001054]
- Korea Science and Engineering Foundation (KOSEF) [R15-2008-006-03002-0]
- GIST
- Korea government (MEST) [2009-0077005]
- Korea Institute of Science and Technology Information [KSC-2009-S01-0015]
- Kwangwoon University
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG000225] Funding Source: NIH RePORTER
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Fully stretched DNA molecules are becoming a fundamental component of new systems for comprehensive genome analysis. Among a number of approaches for elongating DNA molecules, nanofluidic molecular confinement has received enormous attentions from physical and biological communities for the last several years. Here we demonstrate a well-optimized condition that a DNA molecule can stretch almost to its full contour length: the average stretch is 19.1 mu m +/- 1.1 mu m for YOYO-1 stained lambda DNA ( 21.8 mu m contour length) in 250 nm x 400 nm channel, which is the longest stretch value ever reported in any nanochannels or nanoslits. In addition, based on Odijk's polymer physics theory, we interpret our experimental findings as a function of channel dimensions and ionic strengths. Furthermore, we develop a Monte Carlo simulation approach using a primitive model for the rigorous understanding of DNA confinement effects. Collectively, we present a more complete understanding of nanochannel confined DNA stretching via the comparisons to computer simulation results and Odijk's polymer physics theory.
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