Journal
LAB ON A CHIP
Volume 11, Issue 18, Pages 3106-3112Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20407f
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Funding
- U.S. National Institute for Environmental Health Sciences (NIEHS) through trans-NIH Genes, Environment and Health Initiative [U54ES016115]
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [U54ES016115] Funding Source: NIH RePORTER
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A microfluidic device for solid-phase immunoassays based on microparticle labeling is developed using microvalve-control structures for automated sample processing. Programmable microvalve control in a multilayer structure provides automated sample delivery, adjustable hydrodynamic washing and compatibility with a wide range of substrates. Capture antibodies are derivatized on glass surfaces within the processor using an APTES patterning method, and magnetic microspheres conjugated with a secondary detection antibody are used as labels in a capture-sandwich format. In this microfluidic processor, washing force can be precisely controlled to remove the nonspecifically bound microparticles. Automated microfluidic immunoassays are demonstrated for mouse immunoglobulin (IgG) and human prostate specific antigen (PSA) with limits of detection of 1.8 and 3 pM, respectively. The sample processor architecture is easily parallelized for high-throughput analysis and easily interfaced with various assay substrates.
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