Journal
LAB ON A CHIP
Volume 11, Issue 24, Pages 4274-4278Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20758j
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Funding
- NIH/NIBIB [1RC1EB010593]
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [RC1EB010593] Funding Source: NIH RePORTER
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Most laboratory assays take advantage of multi-step protocols to achieve high performance, but conventional paper-based tests (e. g., lateral flow tests) are generally limited to assays that can be carried out in a single fluidic step. We have developed two-dimensional paper networks (2DPNs) that use materials from lateral flow tests but reconfigure them to enable programming of multi-step reagent delivery sequences. The 2DPN uses multiple converging fluid inlets to control the arrival time of each fluid to a detection zone or reaction zone, and it requires a method to disconnect each fluid source in a corresponding timed sequence. Here, we present a method that allows programmed disconnection of fluid sources required for multi-step delivery. A 2DPN with legs of different lengths is inserted into a shared buffer well, and the dropping fluid surface disconnects each leg at in a programmable sequence. This approach could enable multi-step laboratory assays to be converted into simple point-of-care devices that have high performance yet remain easy to use.
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