Journal
LAB ON A CHIP
Volume 11, Issue 15, Pages 2591-2597Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20085b
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Funding
- Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) [R0A-2008-000-20078-0]
- Carbon Dioxide Reduction & Sequestration Research Center [DG2-201]
- Ministry of Science and Technology of Korean government of the Republic of Korea
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Herein, we present the use of a single gold nanorod sensor for detection of diseases on an antibody-functionalized surface, based on antibody-antigen interaction and the localized surface plasmon resonance (LSPR) lambda(max) shifts of the resonant Rayleigh light scattering spectra. By replacing the cetyltrimethylammonium bromide (CTAB), a tightly packed self-assembled monolayer of HS (CH2)(11)(OCH2CH2)(6)OCH2COOH(OEG(6)) has been successfully formed on the gold nanorod surface prior to the LSPR sensing, leading to the successful fabrication of individual gold nanorod immunosensors. Using prostate specific antigen (PSA) as a protein biomarker, the lowest concentration experimentally detected was as low as 111 aM, corresponding to a 2.79 nm LSPR lambda(max) shift. These results indicate that the detection platform is very sensitive and outperforms detection limits of commercial tests for PSA so far. Correlatively, its detection limit can be equally compared to the assays based on DNA biobarcodes. This study shows that a gold nanorod has been used as a single nanobiosensor to detect antigens for the first time; and the detection method based on the resonant Rayleigh scattering spectrum of individual gold nanorods enables a simple, label-free detection with ultrahigh sensitivity.
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