Journal
LAB ON A CHIP
Volume 10, Issue 12, Pages 1518-1520Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c001501f
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Funding
- Monash University
- Australian NHMRC [381413]
- ARC [DP 0773221]
- NHMRC [508927]
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A new method for in-gel sample processing and tryptic digestion of proteins is described. Sample preparation, rehydration, in situ digestion and peptide extraction from gel slices are dramatically accelerated by treating the gel slice with surface acoustic waves (SAWs). Only 30 minutes total workflow time is required for this new method to produce base peak chromatograms (BPCs) of similar coverage and intensity to those observed for traditional processing and overnight digestion. Simple set up, good reproducibility, excellent peptide recoveries, rapid turnover of samples and high confidence protein identifications put this technology at the forefront of the next generation of proteomics sample processing tools.
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