Journal
LAB ON A CHIP
Volume 10, Issue 21, Pages 2986-2993Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c005029f
Keywords
-
Categories
Funding
- NSF [CBET-0643583]
- NIH [NIGMS R01GM090207]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM090207] Funding Source: NIH RePORTER
Ask authors/readers for more resources
We report on measurements of the volume growth rate of ten individual budding yeast cells using a recently developed MOSFET-based microfluidic Coulter counter. The MOSFET-based microfluidic Coulter counter is very sensitive, provides signals that are immune from the baseline drift, and can work with cell culture media of complex composition. These desirable features allow us to directly measure the volume growth rate of single cells of Saccharomyces cerevisiae LYH3865 strain budding yeast in YNB culture media over a whole cell cycle. Results indicate that all budding yeast follow a sigmoid volume growth profile with reduced growth rates at the initial stage before the bud emerges and the final stage after the daughter gets mature. Analysis of the data indicates that even though all piecewise linear, Gomperitz, and Hill's function models can fit the global growth profile equally well, the data strongly support local exponential growth phenomenon. Accurate volume growth measurements are important for applications in systems biology where quantitative parameters are required for modeling and simulation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available