Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that nonmotile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 +/- 4.2 mu m s(-1), while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of -0.8 +/- 1.0 mu m s(-1). Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.