Journal
LAB ON A CHIP
Volume 10, Issue 23, Pages 3210-3212Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00161a
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Funding
- European Community [37957]
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Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.
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