Journal
LAB ON A CHIP
Volume 10, Issue 10, Pages 1267-1273Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b924151e
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Funding
- EPSRC [EP/D073154]
- Insight Faraday Partnership
- SFC
- Lab 901 Ltd
- Japan Society for the Promotion of Science and InvestNI [RTD COE 40]
- Engineering and Physical Sciences Research Council [EP/D073154/2, EP/D073154/1] Funding Source: researchfish
- EPSRC [EP/D073154/1, EP/D073154/2] Funding Source: UKRI
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The use of a water-soluble, thermo-responsive polymer as a highly sensitive fluorescence-lifetime probe of microfluidic temperature is demonstrated. The fluorescence lifetime of poly(N-isopropylacrylamide) labelled with a benzofurazan fluorophore is shown to have a steep dependence on temperature around the polymer phase transition and the photophysical origin of this response is established. The use of this unusual fluorescent probe in conjunction with fluorescence lifetime imaging microscopy (FLIM) enables the spatial variation of temperature in a microfluidic device to be mapped, on the micron scale, with a resolution of less than 0.1 degrees C. This represents an increase in temperature resolution of an order of magnitude over that achieved previously by FLIM of temperature-sensitive dyes.
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