Journal
LAB ON A CHIP
Volume 10, Issue 16, Pages 2147-2153Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c004629a
Keywords
-
Categories
Funding
- NIH [1R01GM0773301]
- NIH Director's Pioneer Award program [1DP1OD003584]
- Alexander v. Humboldt Society
- Yen Postdoctoral Fellowship
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM077331] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP1OD003584] Funding Source: NIH RePORTER
Ask authors/readers for more resources
In this article, we developed a plant on a chip'' microfluidic platform that can control the local chemical environment around live roots of Arabidopsis thaliana with high spatial resolution using multi-laminar flow. We characterized the flow profile around the Arabidopsis root, and verified that the shear forces within the device (similar to 10 dyne cm(-2)) did not impede growth of the roots. Our platform was able to deliver stimuli to the root at a spatial resolution of 10-800 mu m. Further, the platform was validated by exposing desired regions of the root with a synthetic auxin derivative, 2,4-dichlorophenoxyacetic acid (2,4-D), and its inhibitor N-1-naphthylphthalamic acid (NPA). The response to the stimuli was observed using a DR5::GFP Arabidopsis line, where GFP expression is coupled to the auxin response regulator DR5. GFP expression in the root matched the position of the flow-focused stream containing 2,4-D. When the regions around the 2,4-D stimulus were exposed to the auxin transport inhibitor NPA, the active and passive transport mechanisms of auxin could be differentiated, as NPA blocks active cell-to-cell transport of auxin. Finally, we demonstrated that local 2,4-D stimulation in a similar to 10 mu m root segment enhanced morphological changes such as epidermal hair growth. These experiments were proof-of-concept and agreed with the results expected based on known root biology, demonstrating that this root on a chip'' platform can be used to test how root development is affected by any chemical component of interest, including nitrogen, phosphate, salts, and other plant hormones.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available