Journal
LAB ON A CHIP
Volume 10, Issue 16, Pages 2062-2070Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c004285d
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Funding
- US Army Medical Research & Materiel Command (USAMRMC) [W81XWH0810701]
- Telemedicine & Advanced Technology Research Center (TATRC) [W81XWH0810701]
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Cell-encapsulated hydrogels with complex three-dimensional (3D) structures were fabricated from photopolymerizable poly(ethylene glycol) diacrylate (PEGDA) using modified 'top-down' and 'bottoms-up' versions of a commercially available stereolithography apparatus (SLA). Swelling and mechanical properties were measured for PEGDA hydrogels with molecular weights (M-w) ranging from 700 to 10 000 Daltons (Da). Long-term viability of encapsulated NIH/3T3 cells was quantitatively evaluated using an MTS assay and shown to improve over 14 days by increasing the M-w of the hydrogels. Addition of adhesive RGDS peptide sequences resulted in increased cell viability, proliferation, and spreading compared to pristine PEG hydrogels of the same M-w. Spatial 3D layer-by-layer cell patterning was successfully demonstrated, and the feasibility of depositing multiple cell types and material compositions into distinct layers was established.
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