☆ 4.7 Article

Counting single molecules in sub-nanolitre droplets

LAB ON A CHIP (2010)

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Journal

LAB ON A CHIP
Volume 10, Issue 2, Pages 161-164

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/b917503b

Keywords

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Categories

Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation

Funding

  1. DARPA sponsoredMicro/Nano Fluidics Fundamentals Focus Center [MF3]
  2. NIH [1R21CA120742-01]
  3. NSF [0546012, 0730503, 0725528]
  4. NATIONAL CANCER INSTITUTE [R21CA120742] Funding Source: NIH RePORTER
  5. NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U54AI057168] Funding Source: NIH RePORTER

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We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.

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