Journal
LAB ON A CHIP
Volume 10, Issue 22, Pages 3157-3162Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00132e
Keywords
-
Categories
Funding
- National Cancer Institute [5U54 CA119347]
- NATIONAL CANCER INSTITUTE [U54CA119347] Funding Source: NIH RePORTER
Ask authors/readers for more resources
We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody barcode arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available