Journal
LAB ON A CHIP
Volume 10, Issue 9, Pages 1113-1119Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b922884e
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Funding
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at the Institute of Molecular Medicine at University of California, Los Angeles
- California Institute of Regenerative Medicine
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP2OD006462] Funding Source: NIH RePORTER
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Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 mg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.
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