Journal
LAB ON A CHIP
Volume 9, Issue 1, Pages 164-166Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b807682k
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We present a microfluidic system for time-lapsed, live cell microscopy with the ability to control solution exchange via a dynamic flow controller. The application specific microfluidic plates are designed to maintain adherent and non-adherent cell types for multiple days with continuous medium perfusion. Upstream channels with flow controlled via custom software allow the delivery of unique exposure profiles to the cultured cells, such as square waves, step functions, ramps, etc.
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