Journal
LAB ON A CHIP
Volume 9, Issue 12, Pages 1732-1739Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b821508a
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Funding
- NASA through Institute for Cell Mimetic for Space Exploration (CMISE)
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We report the integration of two technologies: droplet microfluidics using electrowetting-on-dielectric (EWOD) and individual particle manipulation using optoelectronic tweezers (OET)-in one microfluidic device. The integrated device successfully demonstrates a sequence involving both EWOD and OET operations. We encountered various challenges during integration of the two different technologies and present how they are addressed. To show the applicability of the device in cellular biology, live HeLa cells are used in the experiments. The unique advantages of EWOD and OET make their integration a significant step towards a powerful tool for many applications, such as single cell studies involving multiplexed environmental stimuli.
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