Journal
LAB ON A CHIP
Volume 9, Issue 10, Pages 1344-1348Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b816049j
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Funding
- EC FP6 Marie Curie Research Training Network
- FEBS
- HFSP
- Ministere de I'Enseignement Superieur et de la Recherche
- CNRS
- ANR [ANR-05-BLAN-0397]
- Agence Nationale de la Recherche (ANR) [ANR-05-BLAN-0397] Funding Source: Agence Nationale de la Recherche (ANR)
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Together with droplet creation, fusion and sorting, the incubation of droplets is one of the most important and essential operations for droplet-based microfluidic assays. This manuscript concerns the development of delay-lines, which are necessary to allow incubation of reactions for precise time periods. We analyze the problems associated with creating delay-lines for incubation in the minute to hour time range, which arise from back-pressure and from the dispersion in the incubation time due to the unequal speeds with which droplets pass through the delay-line. We describe delay-line systems which resolve these problems and demonstrate their use to measure reaction kinetics over several minutes in droplets.
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