Journal
LAB ON A CHIP
Volume 9, Issue 16, Pages 2398-2401Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b906257b
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Funding
- Korean Government (MOEHRD) [KRF-2006-214-D00014]
- Institute for Cell Mimetic Space Exploration (CMISE
- NASA University Research, Engineering and Technology Institute (URETI) [NCC 2-1364]
- NSF [DGE0654431]
- National Institutes of Health [RR020070]
- NATIONAL CENTER FOR RESEARCH RESOURCES [R01RR020070] Funding Source: NIH RePORTER
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We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.
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