Journal
LAB ON A CHIP
Volume 9, Issue 21, Pages 3110-3117Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b904724g
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Funding
- EPSRC [EP/E065414/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/E065414/1] Funding Source: researchfish
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An extremely versatile microfluidic device is demonstrated in which multi-step (bio) chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio) reactions across a rectangular reaction chamber. Functionalised magnetic microparticles are employed as mobile solid-supports and are pulled from one side of the reaction chamber to the other by use of an external magnetic field. As the particles traverse the co-laminar reagent streams, binding and washing steps are performed on their surface in one operation in continuous flow. The applicability of the platform was first demonstrated by performing a proof-of-principle binding assay between streptavidin coated magnetic particles and biotin in free solution with a limit of detection of 20 ng mL(-1) of free biotin. The system was then applied to a mouse IgG sandwich immunoassay as a first example of a process involving two binding steps and two washing steps, all performed within 60 s, a fraction of the time required for conventional testing.
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