Journal
LAB ON A CHIP
Volume 9, Issue 15, Pages 2217-2223Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b900035f
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Funding
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research (MEXT)
- Research and Development Program for New Bio-industry Initiatives
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We present a method for forming monodisperse semi-permeable microcapsules composed of an alginate-poly-L-lysine (PLL) membrane for the observation of encapsulated cells. These microcapsules were prepared with a monolithic three-dimensional microfluidic axisymmetric flow-focusing device by an internal gelation method using glucono-1,5-lactone in order to provide mild conditions for the cells. The microcapsules were sufficiently monodisperse and robust to be trapped in a bead-based microfluidic array system for easy observation. We also confirmed that (i) the alginate-PLL membrane is semi-permeable so that cells and microorganisms cannot pass through it but nutrients and wastes can, (ii) cells are able to move freely inside the semi-permeable microcapsules, and (iii) cells can be successfully proliferated in the microcapsules.
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