Journal
LAB ON A CHIP
Volume 9, Issue 5, Pages 653-656Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b816198d
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Funding
- National Human Genome Research Institute [NSRA F32HG003100]
- Burroughs Wellcome Fund
- [NIH R41 AI060057]
- [NIH R21 CA 127884]
- NATIONAL CANCER INSTITUTE [R21CA127884] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [F32HG003100] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R41AI060057] Funding Source: NIH RePORTER
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The influenza virus has been subtyped from crude lysates using polymerization-based amplification. In this novel chemical approach to detection, signal amplification was achieved by coupling a polymerization reaction to a protein-protein recognition event. This particular method shows promise due to its advantages over the techniques currently employed in commercial assays in terms of cost, robustness, and unambiguity of test results. Instrumentation is not required to see the crosslinked hydrogel readout, and no false positives or false negatives were observed above the limit of detection.
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