Journal
LAB ON A CHIP
Volume 9, Issue 6, Pages 756-760Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b821265a
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Funding
- National Basic Research Program of China [2007CB925101]
- NSFC [20721002, 20827001, 20571042]
- State key laboratory of bioelectronics, Southeast University
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A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation ( ATRP) polymerisation of ( polyethylene glycol) methacrylate (PEGMA) with surface Si-H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.
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