Journal
LAB ON A CHIP
Volume 8, Issue 11, Pages 1888-1895Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b809892a
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Funding
- DOE [DE-FG02-OOER45852]
- NSF-IGERT Biomechanics Training Grant [DGE-0221682]
- NDSEG Fellowship
- Novartis Foundation
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This paper describes the fabrication of a composite agar/PDMS device for enriching short cells in a population of motile Escherichia coli. The device incorporated ratcheting microchannels, which directed the motion of swimming cells of E. coli through the device, and three sorting junctions, which isolated successively shorter populations of bacteria. The ratcheting microchannels guided cells through the device with an average rate of displacement of (32 +/- 9) mu m s(-1). Within the device, the average length of the cells decreased from 3.8 mu m (Coefficient of Variation, CV: 21%) at the entrance, to 3.4 mu m (CV: 16%) after the. first sorting junction, to 3.2 mu m (CV: 19%) after the second sorting junction, to 3.0 mu m (CV: 19%) after the third sorting junction.
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