Journal
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
Volume 15, Issue 6, Pages 415-422Publisher
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
DOI: 10.4196/kjpp.2011.15.6.415
Keywords
Oscillatory rhythmic activity; Retinal ganglion cell spike; Local field potential; rd1 mice; rd10 mice
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Funding
- Ministry of Health Welfare [A050251]
- MEST [2009-0065444, 2010-0020852]
- Chungbuk National University
- National Research Foundation of Korea [2009-0065444, 2010-0020852] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Previously, we reported that besides retinal ganglion cell (RGC) spike, there is similar to 10 Hz oscillatory rhythmic activity in local field potential (LFP) in retinal degeneration model, rd1 mice. The more recently identified rd10 mice have a later onset and slower rate of photoreceptor degeneration than the rd1 mice, providing more therapeutic potential. In this study, before adapting rd10 mice as a new animal model for our electrical stimulation study, we investigated electrical characteristics of rd10 mice. From the raw waveform of recording using 8x8 microelectrode array (MEA) from in vitro-whole mount retina, RGC spikes and LFP were isolated by using different filter setting. Fourier transform was performed for detection of frequency of bursting RGC spikes and oscillatory field potential (OFP). In rd1 mice, similar to 10 Hz rhythmic burst of spontaneous RGC spikes is always phase-locked with the OFP and this phase-locking property is preserved regardless of postnatal ages. However, in rd10 mice, there is a strong phase-locking tendency between the spectral peak of bursting RGC spikes (similar to 5 Hz) and the first peak of OFP (similar to 5 Hz) across different age groups. But this phase-locking property is not robust as in rd1 retina, but maintains for a few seconds. Since rd1 and rd10 retina show phase-locking property at different frequency (similar to 10 Hz vs. similar to 5 Hz), we expect different response patterns to electrical stimulus between rd1 and rd10 retina. Therefore, to extract optimal stimulation parameters in rd10 retina, first we might define selection criteria for responding rd10 ganglion cells to electrical stimulus.
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