Journal
KOREAN JOURNAL OF CHEMICAL ENGINEERING
Volume 27, Issue 2, Pages 587-589Publisher
KOREAN INSTITUTE CHEMICAL ENGINEERS
DOI: 10.1007/s11814-010-0100-3
Keywords
S-adenosyl-L-methionine; Saccharomyces cerevisiae sake; Metabolic Engineering
Funding
- Korea Research Foundation Grant funded by the Korean Government [KRF-2008-331-130003]
- Yang Young Foundation
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In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.
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