Journal
KIDNEY INTERNATIONAL
Volume 80, Issue 9, Pages 926-937Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ki.2011.196
Keywords
cell and transport physiology; immunohistochemistry; intracellular pH; ion transport; physiology
Categories
Funding
- Department of Veterans Affairs
- National Institute of Health [DK 62809]
- US Renal Care, DCA
- DCI dialysis care groups
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Chloride has an important role in regulating vacuolar H+-ATPase activity across specialized cellular and intracellular membranes. In the kidney, vacuolar H+-ATPase is expressed on the apical membrane of acid-secreting A-type intercalated cells in the collecting duct where it has an essential role in acid secretion and systemic acid base homeostasis. Here, we report the identification of a chloride transporter, which co-localizes with and regulates the activity of plasma membrane H+-ATPase in the kidney collecting duct. Immunoblotting and immunofluorescent labeling identified Slc26a11 (similar to 72 kDa), expressed in a subset of cells in the collecting duct. On the basis of double-immunofluorescent labeling with AQP2 and identical co-localization with H+-ATPase, cells expressing Slc26a11 were deemed to be distinct from principal cells and were found to be intercalated cells. Functional studies in transiently transfected COS7 cells indicated that Slc26a11 (designated as kidney brain anion transporter (KBAT)) can transport chloride and increase the rate of acid extrusion by means of H+-ATPase. Thus, Slc26a11 is a partner of vacuolar H+-ATPase facilitating acid secretion in the collecting duct. Kidney International (2011) 80, 926-937; doi:10.1038/ki. 2011.196; published online 29 June 2011
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